| Kevin James | ... Otis the Cow |
| Courteney Cox | ... Daisy the Cow |
| Sam Elliott | ... Ben the Cow |
| Danny Glover | ... Miles the Mule |
| Wanda Sykes | ... Bessy the Cow |
| Andie MacDowell | ... Etta the Hen |
| David Koechner | ... Dag the Coyote |
|
| Jeff Garcia | ... Pip the Mouse |
| Cam Clarke | ... Freddy the Ferret |
| Rob Paulsen | ... Peck the Rooster |
| Tino Insana | ... Pig the Pig |
| Dom Irrera | ... Duke the Dog |
|
| Scott Bullock | ... Eddy the Cow |
| John DiMaggio | ... Bud the Cow |
| Maurice LaMarche | ... Igg the Cow |
| Madeline Lovejoy | ... Maddy the Chick |
| Nathaniel Stroman | ... Root the Rooster |
| Steve Oedekerk | ... Snotty Boy |
| Maria Bamford | ... Mrs. Beady |
| Steve Oedekerk | ... Mr. Beady |
| John DiMaggio | ... Officer O'Hanlon |
| Fred Tatasciore | ... Farmer |
|
| Rob Paulsen | ... Gopher |
| Peter Stenstrom | ... Skunk |
| Steve Oedekerk | ... Snotty Boy's Father |
| Jill Talley | ... Snotty Boy's Mother |
| Laraine Newman | ... Snotty Boy's Friend |
| Katie Leigh | ... Snotty Boy's Friend |
| North Mississippi Allstars | ... Barn Boys |
| Anna Pistor | ... Chick |
| Thomas Pistor | ... Chick |
| Eliana Bendetson | ... Chick |
| Paul Butcher | ... Chick |
| Khamani Griffin | ... Chick |
| Arlo Levin | ... Chick |
| Liliana Mumy | ... Chick |
| Cydney Neal | ... Chick |
| Cate Ozawa | ... Chick |
| Isaiah Tefilo | ... Chick |
| Georgia Van Newkirk | ... Chick |
| Joni Allen | ... Singer |
|
| Additional Barnyard Voices |
| Leigh French | ... Additional Barnyard Voices |
| William Calvert | ... Additional Barnyard Voices |
| Julianne Buescher | ... Additional Barnyard Voices |
| Nicholas Guest | ... Additional Barnyard Voices |
| Audrey Wasilewski | ... Additional Barnyard Voices |
| Kate Leigh | ... Additional Barnyard Voices |
| Philip Proctor | ... Additional Barnyard Voices |
| Archie Hahn | ... Additional Barnyard Voices |
| Chad Einbinder | ... Additional Barnyard Voices |
| Keith Anthony | ... Additional Barnyard Voices |
| Claudette Wells | ... Additional Barnyard Voices |
| Lynne Marie Stewart | ... Additional Barnyard Voices |
| Christie Mellor | ... Additional Barnyard Voices |
| Justin Shenkarow | ... Additional Barnyard Voices |
| Jacqueline Pinol | ... Additional Barnyard Voices |
| Nika Futterman | ... Additional Barnyard Voices |
| Eddie Frierson | ... Additional Barnyard Voices |
